Tasleem Javaid

Research Scientist

Post Doctoral Associate

• Work history includes basic and applied research in plant science including collaborations and training experience at Ohio State University. Skills list includes but is not limited to enzymatic manipulation of DNA and RNA, PCR, construction of CRISPR-CAS9 cassette, E.coli and Agro transformation, bacterial expression and purification of proteins, SDS-PAGE, Southerns, northerns, westerns, protein-protein interaction, enzyme assays, confocal microscopy and compound microscopy. Tissue culture techniques, Rice and Duckweed calli induction, CRISPR generated transgenic plants.

• Good communication skills with experience in public speaking and writing for both technical and non-technical audiences.

• Supervisory experience; training and advising graduate and undergraduate students.

• Outstanding academic performance includes Ph.D. in Environmental & plant biology, MS in plant Biology, and a BS in plant biology. .

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What I Do

Molecular Biology

Gene cloning, overlap PCR, DNA and RNA extraction, quantitative real- time RT-PCR, CRISPR-CAS9 gene editing technology, Co-expression of multiple genes in Tobacco epidermal cells, Agrobacterium-mediated Rice callus genetic transformation, Arabidopsis floral dip transformation.

Cell Biology

Confocal Microscopy, Compound Microscopy

Plant Tissue Culture

Generation of Calli from rice seeds and Duckweed; regeneration of plants from callus, Rice Calli Agrobacterium-mediated transformation.

Protein biochemistry

Protein purification; Enzyme activity assays: Microsome preparation: SDS-PAGE: Western blotting.

Resume

10 Years of Experience

Education

December 2022

Ohio University

Ph.D., Plant Biology

Department of Environmental & Plant Biology, Ohio University, Athens, Ohio, USA 45701

April 2015

University of the Punjab

Master of Science, Botany

Department of Botany, University of the Punjab, Lahore, Punjab, Pakistan 54590

July 2012

University of the Punjab

Bachelor of Science, Botany

Department of Botany, University of the Punjab, Lahore, Punjab, Pakistan 54590

TECHNICAL SKILLS

Molecular Biology

98%

Cell Biology

97%

Plant Tissue culture

96%

Protein biochemistry

99%

Experience

2023 - Present

Complex Carbohydrate Research Center (CCRC)

Post-Doctoral Research Associate

Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center (CCRC), University of Georgia, Athens, Georgia, USA

2021 - 2022

Ohio University

Research Assistant

Department of Environmental and Plant Biology, Ohio University, Athens, Ohio, USA

2017 - 2021

Ohio University

Teaching Assistant

Department of Environmental and Plant Biology, Ohio University, Athens, Ohio, USA

2015 - 2016

Government Post Graduate College for women

Lecturer in Botany

Government Post Graduate College for women, Higher Education Department, Lahore, Punjab, Pakistan

2014 - 2015

Government College of Science

College Teacher Intern (CTI)

Government College of Science, Lahore, Punjab, Pakistan

PUBLICATIONS

2024

The Plant Journal

Specific protein interactions between rice members of the GT43 and GT47 families form various central cores of putative xylan synthase complexes.

Javaid, T., Bhattarai, M., Venkataraghavan, A., Held, M., & Faik, A. (2024). Specific protein interactions between rice members of the GT43 and GT47 families form various central cores of putative xylan synthase complexes. The Plant Journal.

2024

Journal of Biological Chemistry

Streamlining assays of glycosyltransferases activity using in vitro GT-array (i-GT-ray) platform: Application to family GT37 fucosyltransferases

Bhattarai, M., Wang, Q., Javaid, T., Venkataraghavan, A., Hassan, T., O’Neil, M., Tan, L., Chen, H., & Faik, A. (2024). Streamlining assays of glycosyltransferases activity using in vitro GT-array (i-GT-ray) platform: Application to family GT37 fucosyltransferases. Journal of Biological Chemistry.

DISSERTATION

December 10, 2022

The Plant Journal

Xylan Biosynthesis in grasses: Uncovering specific protein-protein interactions (PPI) between rice members of the GT43 and GT47 families and their implication in plant development, Ohio University, December 10, 2022. ORCID ID=0000-0002-2982-4201.

Tasleem Javaid (2022). Xylan Biosynthesis in grasses: Uncovering specific protein-protein interactions (PPI) between rice members of the GT43 and GT47 families and their implication in plant development, Ohio University, December 10, 2022. ORCID ID=0000-0002-2982-4201.

POSTERS PRESENTED

August 4-9, 2023

American Society of Plant Biology (ASPB), Savannah Convention Center

Functional characterization of glycosyltransferases in duckweed to enable predictive biology

Tasleem Javaid, Pradeep K. Prabhakar, Digantkumar G. Chapla, Charles J. Corulli, Brayden P. Smith, Samantha Hennen, Morgan J. Willis, Samantha J. Ziegler, Vivek S. Bharadwaj, Kelley W Moremen, Maria J. Peña, Yannick J. Bomble and Breeanna R. Urbanowicz (2023) Functional characterization of glycosyltransferases in duckweed to enable predictive biology. presented at American Society of Plant Biology (ASPB), Savannah Convention Center, August 4-9, 2023.

April 8, 2022

Student Expo, Ohio University

Xylan synthesis mechanism: matching partners.

Tasleem Javaid and Ahmed Faik (2022) Xylan synthesis mechanism: matching partners. presented at Student Expo, Ohio University, April 8, 2022.

April 8, 2022

Student Expo, Ohio University

Use of CRISPR/Cas9 technology to determine the function of three glycosyltransferases possibly involved in xylan biosynthesis in rice.

Helena Littler, Akshayaa Venkataraghavan, Matrika Bhattarai, Tasleem Javaid, and Ahmed Faik (2017) Use of CRISPR/Cas9 technology to determine the function of three glycosyltransferases possibly involved in xylan biosynthesis in rice. presented at Student Expo, Ohio University, April 8, 2022.

April 8, 2022

Student Expo, Ohio University

Predicting protein-protein interactions between glycosyltransferases

Mohsin Ali Nasir, Ramin Mehrani, Tasleem Javaid, Sumit Sharma, Justin Holub, and Ahmed Faik (2017) Predicting protein-protein interactions between glycosyltransferases. presented at Student Expo, Ohio University, April 8, 2022.

July 7, 2019

Cell Wall (XV) meeting, Cambridge, UK

Gene association network-(GAN) guided approach to study xylan synthesis in grasses.

Tasleem Javaid and Ahmed Faik (2019) Gene association network-(GAN) guided approach to study xylan synthesis in grasses. presented at Cell Wall (XV) meeting, Cambridge, UK, July 7, 2019.

April 11, 2019

Student Expo, Ohio University

Genome Editing to determine the physiological function of xylan during rice grain development.

Tasleem Javaid and Ahmed Faik (2019) Genome Editing to determine the physiological function of xylan during rice grain development. presented at Student Expo, Ohio University, April 11, 2019.

July 13, 2018

American Society of Plant Biology (ASPB), Montreal, Canada

Multi-faceted approach to characterize Xylan Synthase Complexes (XSCs) in Rice.

Tasleem Javaid and Ahmed Faik (2018) Multi-faceted approach to characterize Xylan Synthase Complexes (XSCs) in Rice. presented at American Society of Plant Biology (ASPB), Montreal, Canada, July 13, 2018.

July 13, 2018

American Society of Plant Biology (ASPB), Montreal, Canada

Investigating the assembly and trafficking of Xylan Synthase Complexes (XSCs) in Rice.

Ahmed Faik, Tasleem Javaid, Yadi Zhou and Michael Held (2018) Investigating the assembly and trafficking of Xylan Synthase Complexes (XSCs) in Rice. presented at American Society of Plant Biology (ASPB), Montreal, Canada, July 13, 2018.

April 4, 2018

Student Expo, Ohio University

Approaches towards Characterization of Xylan Synthase Complexes (XSCs) in Rice.

Tasleem Javaid and Ahmed Faik (2018) Approaches towards Characterization of Xylan Synthase Complexes (XSCs) in Rice. presented at Student Expo, Ohio University, April 4, 2018.

April 4, 2018

Student Expo, Ohio University

Implementing CRISPR/Cas9 Technology to Investigate Xylan Biosynthesis in Rice.

Kyle Beck, Tasleem Javaid and Ahmed Faik (2018) Implementing CRISPR/Cas9 Technology to Investigate Xylan Biosynthesis in Rice. presented at Student Expo, Ohio University, April 4, 2018.

March 3 - 4, 2018

Midwest ASPB meeting, Iowa State University, Iowa (US)

Approaches towards Characterization of Xylan Synthase Complexes (XSCs) in Rice.

Tasleem Javaid and Ahmed Faik (2018) Approaches towards Characterization of Xylan Synthase Complexes (XSCs) in Rice. presented at Midwest ASPB meeting, Iowa State University, Iowa (US) from March 3-4, 2018.

September 22, 2017

Graduate Research Symposium, Ohio University

Characterization of Xylan Synthase Complexes (XSCs) in Rice cultivar.

Tasleem Javaid and Ahmed Faik (2017) Characterization of Xylan Synthase Complexes (XSCs) in Rice cultivar. presented at Graduate Research Symposium, Ohio University, September 22, 2017.

April 4, 2017

Norman S. Cohn Symposium, Ohio University

Characterization of Xylan Synthase Complexes (XSCs) in Rice cultivar.

Tasleem Javaid and Ahmed Faik (2017) Characterization of Xylan Synthase Complexes (XSCs) in Rice cultivar. presented at Norman S. Cohn Symposium, Ohio University, September 9, 2017. presented at Student Expo, Ohio University, April 4, 2017.

April 4, 2017

Student Expo, Ohio University

Developing CRISPR/Cas9 as a functional genomics tool to study cell wall biosynthesis in rice (Oryza sativa).

Kyle Beck, Caleb Cole, Tasleem Javaid and Ahmed Faik (2017) Developing CRISPR/Cas9 as a functional genomics tool to study cell wall biosynthesis in rice (Oryza sativa). presented at Student Expo, Ohio University, April 4, 2017.

TEACHING EXPERIENCE

2014 - 2015

Government College of Science, Lahore, Punjab, Pakistan

Introduction to Cell Biology

2015 - 2016

Government Post Graduate College for women, Higher Education department, Lahore, Punjab, Pakistan

Introduction to Plant Biology

Fall 2016 - Spring 2017

Environmental and Plant Biology, Ohio University

BIOL 1010: Principles of Biology

Fall 2021

Environmental and Plant Biology, Ohio University.

PBIO 4500/5500: Grader for Biotechnology & genetic engineering

Fall 2019

Environmental and Plant Biology, Ohio University

PBIO 1140 Lab: Foundations of Plant Biology

Spring 2017, 2018, 2019, 2020

Environmental and Plant Biology, Ohio University

PBIO 5240 Lab: Plant Physiology

Spring 2018, 2020, 2022

Environmental and Plant Biology, Ohio University

PBIO 5010 Lab: Laboratory in Cellular and Molecular Plant Physiology

MENTORING

Fall 2017 - 2018

Trained and supervised Kyle Beck and Caleb Cole for their undergraduate research project in Faik lab

Fall 2023 - Spring 2024

Supervised Maria Harman (Undergraduate lab assistant)

Fall 2023

Mentored Nathan Murray (Graduate student), a rotation student in Breeanna Urbanowicz Lab

SERVICE

December 13 - 14, 2023

Volunteer for organizing Plant Center Annual Retreat at Brasstown Valley Resort in Young Harris, Georgia

August 5, 2023

Volunteer at Plant Science Saturday organized by ASPB (held at Savannah convention Center 2023) at Forsyth Farmers Market

Fall 2021 - Fall 2022

Mentored Nathan Murray (Graduate student), a rotation student in Breeanna Urbanowicz Lab

Spring 2018 & 2019

Served as a review committee member for College of Arts and Sciences, Graduate Student Research Funds

Fall 2020

Served as a review committee member for College of Arts and Sciences, Graduate Student Research Funds.

Spring 2018 - 2019

Judging district science fair Spring 2018 & 2019 held at Clippinger, Ohio University, Athens, OH.

Fall 2018 - 2019

Judging posters for Ohio State University Plant Sciences Symposium Spring 2019 Judging middle school science fair.

Spring 2019

Organization teamwork at student expo at Ohio University.

Summer 2019

Participating in teaching children at Morrison Gordon School, Athens, Ohio about plants on Earth Day.

Fall 2019 - Spring 2021

Plant Biology Graduate Student Association (PGSA) Secretary from Fall 2019 to Spring 2021 Faik lab manager from 2017 to 2022.

PROFESSIONAL DEVEOLPMENT

May 2016

Chemical Hygiene Training at Ohio University

July 2017

Radiation Safety Training at Ohio University

November 2019

Electronic Teaching Portfolios Training at Ohio University

MEMBERSHIP IN SCIENTIFIC SOCIETIES

March 2018

Member of American Society of Plant Biologists (ASPB), USA

2014

Member of National Academy of Young Scientists (NAYS), Pakistan

November 2019

Electronic Teaching Portfolios Training at Ohio University

REFERENCES

Dr. Breeanna Urbanowicz

Assistant Professor, Department of Biochemistry and Molecular Biologist at Complex Carbohydrate Research Center (CCRC), University of Georgia, Tel: 706-542-4479, breeanna@uga.edu

Dr. Ahmed Faik

Associate Professor, Department of Environmental and Plant Biology, Ohio University, Tel: 740-593-1121, faik@ohio.edu

Dr. Michael Held

Associate Professor, Department of Chemistry, Ohio University, Tel: 740-593-1751, held@ohio.edu